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Molecular and phylogenic identification of Gammarus komareki species (Gammaridae) from the Jajrood River using DNA barcoding method
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Siamak Yousefi Siahkalroudi    1, Hadi Yousefi Siahkalroudi1 , Farideh Chenari2 , Shadi Khatami1 |
1- Islamic Azad University
2- Protein Gostar Sina co |
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Abstract: (2048 Views) |
Gammarus (Crustacea: Amphipoda) are Gammaridae family that play an important role in food chain, environmental refining of organic matter and high sensitivity to environmental contamination. Therefore, it is important to identify them. Since the identification of the genus Gammarus is very difficult due to similar morphological features, the aim of the present study was to molecular identification and investigation of the phylogenetic relationships of G. komareki from the Jajrood River, east of Tehran compared to other parts of Iran and the rest of the world. For this purpose, sampling of G. komareki species in Jajrood River was done in season from October 2013 to July 2014. Samples were collected by a small manual tour. For molecular studies, some tissue was isolated from each sample with scalpel and fixed in 95% ethyl alcohol. The DNA of the samples was then extracted using the CTAB method and the gene fragment of a mitochondrial cytochrome oxidase (COI) subunit was amplified and sequenced. After gene fragment amplification and sequencing, Neighbor Joining and Maximum Likelihood trees were plotted using MEGA7 software. The results of the present study showed that although the specimens were morphologically belonging to Gammarus komareki species reported from other parts of Iran, Molecular studies showed that the samples of the present study were divided into two categories by being placed in two different clouds. Therefore, it is possible that these specimens may belong to separate species, which needs further investigation with other genetic markers.
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| Keywords: Genetic diversity, Gammarus, Jajrood, The east of Tehran, Iran |
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Full-Text [PDF 884 kb]
(589 Downloads)
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Type of Study: Research |
Subject:
بيوسيستماتيك آبزيان
Received: 2019/11/14 | Accepted: 2020/04/13 | Published: 2020/08/31
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